The high-throughput crystallization hub is integrated with the adjoining macromolecular X-ray analysis hub. A critical step in determining three-dimensional structures of proteins is the generation of crystals of the protein studied. Despite significant progress in the methods of crystallization, crystal generation remains a trial and error process, requiring analysis of thousands of conditions. The platform provides the infrastructure necessary for high-throughput analysis of hundreds of simultaneous crystallization conditions, with robots for dispensing drops of crystallization, a storage station, viewing with Internet access and equipment for the protein analysis. The current instrumentation will be upgraded next year with the addition of a third liquid handling robot and a second state-of-the-art incubator with in situ UV imaging of growing protein crystals.